Deciphering the molecular events that must occur to allow an existing memory to be modified via reconsolidation updating; leveraging this information so rational strategies can be developed that will increase the efficacy of reconsolidation blockade based therapies.
Dr. Jonathan Ploski focuses his research on developing clinically effective methods to therapeutically attenuate maladaptive emotional memories. He recently has been examining how the NMDA receptor subunit composition can influence the ability of an existing memory to be modified via reconsolidation updating. Specifically, Dr. Ploski has developed a line of transgenic mice that could specifically overexpress the GluN2A subunit of the NMDA receptor after a memory has been formed. Subsequently, he has found that when the GluN2A subunit is overexpressed, the modification of an existing memory is prevented. Dr. Ploski also has developed a viral-based, inducible CRISPR/Cas9 system for in vivo genome editing that can be used for studies focused on the role of specific genes and behavior. His lab has received a seed grant from the Texas Medical Device Center to develop a viral delivered inducible genome editing system that will facilitate the investigation of how particular genes influence neural plasticity and behavior. He is expecting to receive an NIH/NIMH grant to further develop and enhance this technology this summer. Dr. Ploski received his bachelor’s degree from the University of Buffalo and his PhD from Mount Sinai School of Medicine.
Recent Articles in Peer-Refereed Journals
Increasing the GluN2A/GluN2B Ratio in Neurons of the Mouse Basal and Lateral Amygdala Inhibits the Modification of an Existing Fear Memory Trace. Holehonnur R, Phensy AJ, Kim LJ, Milivojevic M, Vuong D, Daison DK, Alex S, Tiner M, Jones LE, Kroener S, Ploski JE. J Neurosci. 2016 Sep 7;36(36):9490-504. doi: 10.1523/JNEUROSCI.1743-16.2016. PMID: 27605622
The Development of a Viral Mediated CRISPR/Cas9 System with Doxycycline Dependent gRNA Expression for Inducible In vitro and In vivo Genome Editing. de Solis CA, Ho A, Holehonnur R, Ploski JE. Front Mol Neurosci. 2016 Aug 18;9:70. doi: 10.3389/fnmol.2016.00070. eCollection 2016. PMID: 2758799
Overexpression of Homer1a in the basal and lateral amygdala impairs fear conditioning and induces an autism-like social impairment. Banerjee A, Luong JA, Ho A, Saib AO, Ploski JE. Mol Autism. 2016 Feb 29;7:16. doi: 10.1186/s13229-016-0077-9. eCollection 2016. PMID: 26929812