PhD, Mount Sinai School of Medicine
Molecular and Biochemical Mechanisms of Synaptic Plasticity, Emotional Learning and Memory
My research interests are directed toward elucidating the molecular and cellular mechanisms of neuronal plasticity that govern emotional memory as well as identifying the causes and consequences of aberrant forms of plasticity that occur in psychiatric disorders, such as anxiety. Collectively my research utilizes a myriad of molecular, biochemical and behavioral approaches.
My laboratory is currently directing its research efforts toward the molecular basis of memory modification. Memory modification utilizing reconsolidation updating is being examined as one of the potential treatment approaches for attenuating maladaptive memories associated with emotional disorders. However, studies have shown that while weak memories can be modified using reconsolidation updating, strong memories can be resistant to this approach. Therefore, treatments targeting the reconsolidation process are unlikely to be clinically effective unless methods are devised to enhance retrieval-dependent memory destabilization. Currently little is known about the cellular and molecular events that influence the induction of reconsolidation updating. My laboratory has recently determined that that an increase in the GluN2A/GluN2B ratio interferes with retrieval-dependent memory destabilization and inhibits the modification of an existing fear memory trace. We are currently investigating methods to enhance the initiation of reconsolidation updating so strong memories can be therapeutically modified utilizing a variety of pharmacological and molecular genetic approaches.
In the News
Specialties: Pavlovian fear conditioning, memory consolidation and reconsolidation, AAV and Lenti virus production for in vivo delivery of recombinant transgenes, Gene expression analysis, Gene identification, Viral mediated genome editing and associated CRISPR/Cas9 approaches.
Increasing the GluN2A/GluN2B Ratio in Neurons of the Mouse Basal and Lateral Amygdala Inhibits the Modification of an Existing Fear Memory Trace. Holehonnur R, Phensy AJ, Kim LJ, Milivojevic M, Vuong D, Daison DK, Alex S, Tiner M, Jones LE, Kroener S, Ploski JE. J Neurosci. 2016 Sep 7;36(36):9490-504. doi: 10.1523/JNEUROSCI.1743-16.2016. PMID: 27605622
The Development of a Viral Mediated CRISPR/Cas9 System with Doxycycline Dependent gRNA Expression for Inducible In vitro and In vivo Genome Editing. de Solis CA, Ho A, Holehonnur R, Ploski JE. Front Mol Neurosci. 2016 Aug 18;9:70. doi: 10.3389/fnmol.2016.00070. eCollection 2016. PMID: 2758799
Overexpression of Homer1a in the basal and lateral amygdala impairs fear conditioning and induces an autism-like social impairment. Banerjee A, Luong JA, Ho A, Saib AO, Ploski JE. Mol Autism. 2016 Feb 29;7:16. doi: 10.1186/s13229-016-0077-9. eCollection 2016. PMID: 26929812