The Department of Molecular and Cell Biology presents Dr. Andrew D. Smith from the University of Southern California, Divison of Biological Sciences, Molecular and Computational Biology, Los Angeles, CA.
Insights from global analysis of mammalian germ line methylomes and imprinted DNA methylation in somatic cells
Mammalian DNA methylation is an epigenomic mark with the capacity for dynamically encoding and communicating phenotypic information through cell division. Epigenomic reprogramming, which happens in germ cell and embryonic development, functions to reset epigenomic states for proper initiation of developmental programs. I will present results from global analysis of DNA methylation in male germ cells of mammals. These results demonstrate substantial differences between the outcomes of embryonic and germ cell reprogramming, and point to rapid evolution of global methylation features in male germ cells.
While embryonic reprogramming erases most of the methylation on paternal and maternal alleles, imprinted loci retain methylation marks set in parents’ germ cells and regulate parent-of-origin expression patterns in the offspring. I will describe an algorithmic approach to identify imprinted methylation from whole-genome bisulfite sequencing data. By applying this algorithm to somatic methylomes in human and mouse, we have predicted an expanded set of imprinted loci, and a correspondingly expanded role for gene regulation by epigenomic states set parental gametes.
Refreshments will be served at 3:45PM in Suite FO3.606.