WARREN J. GOUX

Associate Professor

University of Texas at Dallas
Department of Chemistry

Phone: (972)883-2660
Fax: (972)883-2925
Email: wgoux@utdallas.edu

Education

Ph.D., Chemistry, 1977, University of California, Santa Barbara.
Physical Chemistry, Biochemistry. Emphasis in conformational and optical properties of amino acids, peptides, proteins and related molecules.
B.A., Psychology, University of Southern California, Los Angeles, California, 1971.

Fields of Research Specialization

Biophysical Chemistry, proteins and glycoproteins, and oligosaccharides.
Pulsed NMR techniques as a means of probing structure and dynamics of biologically relevant molecules.
Enzymatic synthesis of isotopically labeled compounds.

Experience

1986 - Associate Professor of Chemistry, The University of Texas at Dallas.
Summers - Visiting Faculty at Los Alamos National Laboratory.
1983, 85, 87 - Contacts: Robert E. London, Clifford J. Unkefer.

Research Interests

Since coming to UTD my major interest has been to use NMR and isotopically labeled compounds to investigate complex carbohydrate structures of biological interest. I pioneered the use of carbon resonances of complex carbohydrates peracetylated with 13C-labeled reagents to identify their structures. It was necessary to correlate the fingerprint of resonances and coupling constants contained in more than one experiment to the unknown structure. Operationally, this involved the application of pattern recognition methods to select components of the data which would lead to the most accurate selection of substructures. My studies show that this global approach to the identification of carbo-hydrate structure by NMR is superior to previous methods which attempted to identify structures on the basis of the NMR data associated with only one type of proton resonance. Most recently, these methods have been applied to the identification of complex carbohydrates contained in paired helical filaments (PHF) isolated from the brains of Alzheimer's Diseased (AD) patients. It is likely that an understanding of these structures will better our understanding of the underlying causes of AD.

My interest in the use of isotopically labeled compounds has also lead me to new methods for their preparation using enzymes. This work has lead to three patents in the area

Patents

"Enzymatic Synthesis of Isotopically Labeled Carbohydrates from Labeled Pyruvate", U.S. Patent # 4,656,133, 1987.
"Enzymatic Synthesis of Isotopically Labeled Carbohydrates, Nucleotides and Citric Acid Cycle Intermediates", US Patent #5,227,296, July, 1993.
"Enzymatic Synthesis of Isotopically Labeled Carbohydrates", US Patent # 5,302,520, April, 1994

Key Publications

W.J. Goux and C. J. Unkefer, "The Assignment of Carbonyl Resonances in 13C NMR Spectra of Peracetylated Mono- and Oligo-saccharides Containing D- Glucose and D-Mannose: An Alternative Method for Structural Determination Complex Carbohydrates", Carbohydrate Research, 159, 191 (1988).

W.J. Goux, "The Determination of Complex Carbohydrate Structure Using Carbonyl Carbon Resonances of Peracetylated Derivatives", Carbohydr. Res., 184, 47-56 (1989).

W.J. Goux, "NMR Pattern Recognition of Peracetylated Mono- and Oligosaccharide Structures. Classification of Residues Using Principal Component Analysis, K-Nearest Neighbor Analysis and SIMCA Class Modeling", J. Magn. Res., 85, 457-469 (1989).

W.J. Goux, D.S. Weber, and G. Okike, "The Selection of NMR Spectral Features Leading to the Optimum Classification of Residue Types in Peracetylated Oligosaccharide Derivatives", J. Magn. Res., 96, 526-540 (1992).

W. J. Goux, S. J. Hill, C. M. Jones and C. L White, III, with D. R. Sparkman, "Alzheimer Disease Paired Helical Filament Core Structures Contain Glycolipid", Biochem. Biophys. Res. Commun., 181, 771-779 (1991).

W.J. Goux and with D.S. Weber, "The Application of NMR/Pattern Recognition Methods to the Classification of Peracetylated Oligosaccharide Residues: Effects of Intraclass Structure", Carbohydr. Res., 233, 65-80 (1992).

W.J. Goux, K. T. Kuan, D. S. Weber, and L. Sottile, "A Convenient Method for the Preparation of a Variety of 13C-enriched D-Fructose Phosphates Using Readily Available Enzymes of the Glycolytic Pathway", Carbohydr. Res., 225, 123-136 (1992).

W.J. Goux, L. Rench, and D. S. Weber, "Stereoselective Synthesis of Stable Isotope Labeled L-Ā-Amino Acids: The Enzymatic Preparation of 13C-Labeled L-Glutamic Acids", J. Labeled Comp. and Labeled Radiopharm., 38, 181-193 (1993).

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Last modified 19 November 1997