Wavelet-based Preprocessing Methods for Malditof Data
Dr. Jeffrey S. Morris
Associate Professor
The University of Texas, MD Anderson Cancer Center Houston, TX
Tuesday, April 05, 2005
2:00 PM
Location: GR 3.302
Open to the public
Coffee will be served in ECSN 3.106 at 1:30 p.m.
Description
Motivation:
Mass spectrometry yields complex functional data for which the features of scientific interest are peaks. A common two-step approach to analyzing these data involves first extracting and quantifying the peaks, then analyzing the resulting matrix of peak quantifications.
Feature extraction and quantification involves a number of interrelated steps. It is important to perform these steps well, since subsequent analyses condition on these determinations. Also, it is difficult to compare the performance of competing methods for analyzing mass spectrometry data since the true expression levels of the proteins in the population are generally not known.
Results:
We introduce a new method for feature extraction in mass spectrometry data that uses translation-invariant wavelet transforms and performs peak detection using the mean spectrum. We examine the method's performance through examples and simulation, and demonstrate the advantages of using the mean spectrum to detect peaks.
We also describe a new physics-based computer model of mass spectrometry, and demonstrate how one may design simulation studies based on this tool to systematically compare competing methods.
For More Information
Lorre Antoine
phone (972)883-2161
fax (972)883-6622
- Updated: June 7, 2005
