Department of Biological Sciences

School of Natural Sciences and Mathematics

Faculty and Research

Donald Gray, Ph.D.

Donald Gray
Professor Emeritus
FO 3.210 972-883-2513 [email protected]

Education and Professional Affiliations

B.A., Math, Physics, and Chemistry, Susquehanna University
M.S., Molecular Biophysics, Yale University
Ph.D., Molecular Biophysics, Yale University
Member, Biophysical Society
Member, American Association for the Advancement of Science
Member, American Chemical Society
Department Head, 1989-1995; 2004-2007
Retired: 2012


One emphasis of past research has been in biophysical studies of nucleic acids and of single-stranded DNA-binding proteins, such as the gene 5 protein of Ff bacterial viruses and the human replication protein A. Another emphasis has been to relate the nearest-neighbor, and next-nearest-neighbor, properties of DNA oligomers to the effectiveness of antisense DNA control of cellular gene expression.

Recent Publications:

H.-N. Nguyen, L. Zhao, C. W. Gray, D. M. Gray, and T. Xia. 2013. Nucleotides sequestered at different subsite loci within DNA-binding pockets of two OB-fold single-stranded DNA-binding proteins are unstacked to different extents. Biopolymers 99: 484-496.

D. M. Gray. 2012. Circular dichroism of protein-nucleic acid interactions. In: "Comprehensive Chiroptical Spectroscopy," Vol. 2 (Eds. N. Berova, P. L. Polavarapu, K. Nakanishi, and R. W. Woody) John Wiley & Sons, Hoboken, NJ.

H.-N. Nguyen, L. Zhao, C. W. Gray, D. M. Gray, and T. Xia. 2011. Ultrafast fluorescence decay profiles reveal differential unstacking of 2-aminopurine from neighboring bases in single-stranded DNA-binding protein subsites. Biochemistry 50:8989-9001.

D. M. Gray, C. W. Gray, B.-H. Yoo, and T.-F. Lou. 2010. Antisense DNA parameters derived from next-nearest-neighbor analysis of experimental data. BMC Bioinformatics 11:252.

J.-H. Fan, E. Bochkareva, A. Bochkarev, and D. M. Gray. 2009. Circular dichroism spectra and electrophoretic mobility shift assays show that human replication protein A binds and melts intramolecular G-quadruplex structures.  Biochemistry 48:1099-1111.

D. M. Gray, J.-D. Wen, C. W. Gray, R. Repges, C. Repges, G. Raabe, and J. Fleischhauer. 2008. Measured and calculated CD spectra of G-quartets stacked with the same or opposite polarities. Chirality 20:431-440.

T.-C. Mou, M. Shen, S. Abdalla, D. DelaMora, E. Bochkareva, A. Bochkarev, and D. M. Gray. 2006. Effects of ssDNA sequences on non-sequence-specific protein binding. Chirality 18:370-382.

J.-D. Wen, and D. M. Gray. 2004. Selection of genomic sequences that bind tightly to Ff gene 5 protein: primer-free genomic SELEX. Nucl. Acids. Res. 32:e182, pp. 1-10.

B. H. Yoo, E. Bochkareva, A. Bochkarev, T.-C. Mou, and D. M. Gray. 2004. 2'-O-methyl-modified phosphorothioate antisense oligonucleotides have reduced non-specific effects in vitro. Nucl. Acids. Res. 32:2008-2016.

W.-Q. Zou, J. Zheng, D. M. Gray, P. Gambetti, and S. G. Chen. 2004. Antibody to DNA detects scrapie but not normal prion protein. Proc. Natl. Acad. Sci., USA 101:1380-1385.

J. D. Wen, and D. M. Gray. 2004. Ff gene 5 single-stranded DNA-binding protein assembles on nucleotides constrained by a DNA hairpin. Biochemistry 43:2622-2634.

T.-F. Lou, C. W. Gray, and D. M. Gray. 2003. The reduction of Raf-1 protein by phosphorothioate ODNs and siRNAs targeted to the same two mRNA sequences. Oligonucleotides 13:313-324.

T.-C. Mou, M. C. Shen, T. C. Terwilliger, and D. M. Gray. 2003. Binding and reversible denaturation of double-stranded DNA by Ff gene 5 protein. Biopolymers 70:637-648.



  • Updated: February 6, 2006