2:45 p.m. - 4 p.m. Location: RL 3.204
Decoding the Non-Coding Genome in Development and Cancer
Our research focuses on the intersection of transcriptional control with stem cell biology, hematopoiesis, and cancer. We employ epigenetics, functional genomics, genome editing, biochemistry, and mouse genetics to define epigenetic and genetic programs that control stem cell development, and how these processes go awry in cancer progression. Dr. Xu will discuss the molecular and genomic analysis of the in vivo function and in situ organization of transcriptional enhancers and other non-coding elements in hematopoiesis and leukemia. Non-coding regulatory DNA elements including enhancers are commonly recognized by correlative chromatin features, yet the molecular composition and structure-function of the vast majority of these elements remains unknown. This is a major impediment for understanding the coordinated control of gene transcription in normal and diseased cells. Recent advances in genomic engineering and chromatin profiling enable the development of new approaches to identify and characterize locus-specific chromatin interactions. By comprehensive studies of enhancer-regulating proteome and 3D interactome in native chromatin, we aim to advance our mechanistic understanding of the non-coding regulatory genome and genetic variations in human diseases.