2:45 p.m. - 4 p.m. Location: RL 3.204
“From transcription factor binding to function: unraveling the stem cell pluripotency network by repurposing CRISPR/Cas9”
Interactions between transcription factors and their genomic binding sites control gene activity. Despite tremendous progress in mapping the binding sites of transcription factors across the genome, outstanding questions still remain. How does transcription factor binding relate to activity of the target gene? How do individual transcription factor binding sites contribute to specific cellular phenotypes? In my seminar I will present a new method, termed CRISPRd, for the rapid functional analysis of specific transcription factor binding sites. I will show how this new technology can be used to unravel the biological function of stem cell pluripotency transcription factors. I will also discuss our current efforts to expand CRISPRd technology for the genome-wide perturbation of the pluripotency transcription network. I will conclude by presenting the future research directions of my own laboratory to investigate the mechanisms that link the cell division cycle to cellular differentiation in pluripotent cells.